发明名称 |
A BIOPROCESS FOR THE GENERATION OF CELLS DERIVED FROM SPHEROID-FORMING CELLS |
摘要 |
The present inventors identified aggregation of embryonic stem cells and embryoid bodies (EBs) as the cause of the difficulty in generating large numbers of the embryonic stem cells (ES) cell-derived tissues. To counter this, the invention provides a novel bioprocess where aggregation of spheroid-forming cells, such as embryonic stem cells and spheroids, such as EBs is controlled, such as by encapsulation of within a matrix. As a result, EBs can be generated with high efficiency and cultured in high cell density, well-mixed systems. Well-mixed conditions facilitate measurement and control of the bulk media conditions and allow for the use of scalable bioreactor systems for clinical production of tissue. Therefore, the invention enables generation of ES cell-derived tissue on a clinical scale. The invention is also applicable to any spheroid-forming cells and other types of pluripotent cells. |
申请公布号 |
WO03004626(B1) |
申请公布日期 |
2003.07.17 |
申请号 |
WO2002CA01022 |
申请日期 |
2002.07.04 |
申请人 |
ZANDSTRA, PETER, W.;DANG, STEPHEN;CARDION AG |
发明人 |
ZANDSTRA, PETER, W.;DANG, STEPHEN |
分类号 |
A01K67/027;C12M3/02;C12N5/00;C12N5/073;C12N5/0735;C12N15/62 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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