IMPROVEMENTS IN AND RELATING TO THE PROVISION OF DNA INVESTIGATING TOOLS

摘要

A method of evaluating primers for use in the amplification of DNA sequences, a method of producing a mixture of primers for such a purpose and a method of amplifying a plurality of DNA sequences using a mixture of primers is provided in which the evaluation involves performing one or more initial evaluations, obtaining a plurality of SNP sites and related potential primer identities which are passes, generating primers corresponding to those primer identities and conducting a test amplification process using those primers in conjunction with a further evaluation of the results, the pass primers therefrom forming a pool of primer candidates from which one or more primers for use in the amplification of DNA sequences are selected. The invention provides a faster, cheaper and more versatile technique for developing multiplexes, particularly for forensic science type investigations.