| 摘要 |
<p>A DHFR-deficient CHO cell line is transfected with anti-apoptotic gene, a method prepares the DHFR-deficient CHO cell line, and a method produces target proteins using the DHFR-deficient CHO cell line. Protein production using animal cells is limited by the low productivity of animal cells compared to microbial cells. Therefore, inhibition of apoptosis is expected to increase in productivity of target proteins by extending longevity of the transfected CHO cell line and to maintain the molecular integrity of unstable target proteins in a medium by decreasing cell lysis.</p> |
