发明名称 |
PCR PRIMER CONSTRUCTS FOR USE IN AN INTEGRATED SIGNALING SYSTEM |
摘要 |
<p>A method for the detection of a target nucleic acid, which method comprises contacting template nucleic acid from a sample with (i) a signalling system and (ii) a tailed nucleic acid primer having a template binding region and the tail comprising a linker and a target binding region, in the presence of appropriate nucleoside triphosphates and an agent for polymerization thereof, under conditions such that the template binding region of the primer will hybridize to a complementary sequence in the template nucleic acid and be extended to form a primer extension product, separating any such product from the template whereupon the target binding region in the tail of the primer will hybridize to a sequence in the primer extension product corresponding to the target nucleic acid, and wherein any such target specific hybridization causes a detectable change in the signalling system, such that the presence or absence of the target nucleic acid in the sample is detected by reference to the presence or absence of a detectable change in the signalling system.</p> |
申请公布号 |
EP1088102(B1) |
申请公布日期 |
2003.07.16 |
申请号 |
EP19980955781 |
申请日期 |
1998.11.25 |
申请人 |
DXS LIMITED |
发明人 |
GIBSON, NEIL JAMES;LITTLE, STEPHEN;THEAKER, JANE;WHITCOMBE, DAVID MARK |
分类号 |
G01N33/50;C12N15/09;C12Q1/68;G01N33/58;(IPC1-7):C12Q1/68 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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