摘要 |
A 1 kbp length of the 5'-noncoding region of the gene encoding cytochrome P-450sca-2 in the actinomycete Streptomyces carbophilus has transcription promoter activity, which is substrate inducible. When the 1 kbp region is shortened, the transcription activity leads to a constitutive transcription activity and the shortened promoters can be used advantageously in expression systems, especially those expressing P-450sca-2 in the presence of ML-236B to produce pravastatin sodium. |