发明名称 |
Initiation of full mammalian oocyte activation by multiple sperm components |
摘要 |
The invention provides a microinsertion-based, trans-complementation method of in vitro oocyte activation useful to identify properties of sperm-borne oocyte-activating factor(s) (SOAF) and ooplasmic interactions with sperm components at fertilization. The invention provides at least one detergent-insoluble heat-sensitive component of the perinuclear matrix of a spermatozoon (SOAFm) that acts coordinately with at least one heat-stable submembrane sperm component. SOAFm is solubilized in vitro (SOAFs) under reducing conditions similar to those encountered in the ooplasm. By the method of the invention, the failure of heat-inactivated demembranated sperm heads to activate an egg is rescued by coinsertion of SOAFs into an oocyte. SOAFs is protease-sensitive and is liberated from demembranated heads in a temperature-dependent manner that inversely correlates with the ability of demembranated sperm heads to activate oocytes.
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申请公布号 |
US6593139(B1) |
申请公布日期 |
2003.07.15 |
申请号 |
US20000478848 |
申请日期 |
2000.01.07 |
申请人 |
UNIVERSITY OF HAWAII |
发明人 |
YANAGIMACHI RYUZO;PERRY ANTHONY C. F. |
分类号 |
A01K67/027;C07K2/00;C07K14/47;C12N5/02;C12N5/075;(IPC1-7):C12N5/00;C12N15/00;A01K67/00;A01K67/033 |
主分类号 |
A01K67/027 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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