| 摘要 |
A sensor probe is disclosed which can measure the hydrogen peroxide content of a single sample using two oxygen sensors whose electrodes are encased in defined membranes. The oxygen reference sensor is encased in a hydrophobic membrane which prevents the transport of hydrogen peroxide or electrochemical poisons or interferents and isolates the electrodes and an electrolyte fluid surrounding the electrodes from the sample fluid. The hydrogen-peroxide-generated oxygen (HPGO) sensor is also is encased in such a hydrophobic membrane, but has in series with and distally of the hydrophobic membrane a hydrophilic membrane which contains an immobilized enzyme such as catalase, peroxidase or other enzymes of a family which catalyzes the reaction of hydrogen peroxide to oxygen and water. At the HPGO sensor, the hydrogen peroxide is catalyzed to oxygen by the enzyme so that the HPGO sensor measures an enhanced concentration of oxygen relative to the oxygen reference sensor. The signals of each of the oxygen sensors are sent to a summer, which subtracts the equal background oxygen concentration of both, yielding a resultant difference signal representative of the concentration of hydrogen peroxide content of the sample itself. A suitable display or data collection device is used to capture the information in visible or digital form. Methods of use of the device for determining hydrogen peroxide content of human or animal tissues or fluids, or environmental or industrial fluids or fluid-containing materials, are also disclosed.
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