发明名称 |
Directional assembly of large viral genomes and chromosomes |
摘要 |
Full-length, functionally intact genomes or chromosomes are directionally assembled with partial cDNA or DNA subclones of a genome. This approach facilitates the reconstruction of genomes and chromosomes in vitro for reintroduction into a living host, and allows the selected mutagenesis and genetic manipulation of sequences in vitro prior to reassembly into a full length genome molecule for reintroduction into the same or different host. This approach also provides an alternative to recombination-mediated techniques to manipulate the genomes of higher plants and animals as well as bacteria and viruses.
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申请公布号 |
US6593111(B2) |
申请公布日期 |
2003.07.15 |
申请号 |
US20010862847 |
申请日期 |
2001.05.21 |
申请人 |
UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL |
发明人 |
BARIC RALPH S.;YOUNT BOYD |
分类号 |
C07K14/165;C12N5/10;C12N7/00;C12N15/10;C12N15/86;(IPC1-7):C12P21/06 |
主分类号 |
C07K14/165 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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