发明名称 Human tyrosine hydroxylase promoter and uses thereof
摘要 The present invention provides an isolated, purified and characterized human tyrosine hydroxylase (hTH) promoter nucleic acid sequence. The invention further provides a method of selecting TH positive (TH+) cells by preparing a construct comprising a hTH promoter operably linked to a heterologous nucleic acid sequence, for example, green fluorescent protein encoding sequence, and transfecting cells, particularly stem cells, with the construct. The invention also provides a hTH promoter, useful in gene therapeutic applications in driving therapeutic genes or other nucleic acid sequences operably linked to the hTH promoter. Additionally, the invention provides cell lines and transgenic animals expressing a transgene comprising the hTH promoter operably linked to a heterologous sequence, which cell lines and transgenic animals are useful for isolating TH+ cells for transplantation or for screening of therapeutic agents that affect TH+ function. Methods of producing cell lines and transgenic animals also provided.
申请公布号 US2003129170(A1) 申请公布日期 2003.07.10
申请号 US20020215647 申请日期 2002.08.09
申请人 IACOVITTI LORRAINE;KESSLER MARK ALEXANDER 发明人 IACOVITTI LORRAINE;KESSLER MARK ALEXANDER
分类号 A61K38/00;A61K48/00;C12N5/08;C12N9/02;C12N15/65;C12N15/85;C12Q1/68;(IPC1-7):G01N33/567;C07H21/04;C12N9/06 主分类号 A61K38/00
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