| 摘要 |
The present invention relates to improvements in methods of DNA mutation detection using chromatographic methods such as DHPLC. In particular, the invention relates to methods for increasing the level of heteroduplex during a hybridization process between a DNA fragment and a corresponding wild type DNA fragment by including during the hybridization process nitrogen-containing organic compounds such as betaine, trimethylamine N-oxide, and dimethylglycine. In another aspect the inventino provides improved PCR primers for amplifying the DNA to be used in the hybridization. The invention also provides for DNA hybridization kits containing these compounds and primers. |
