| 摘要 |
The present invention is a method for analysis of at least peptides comprisi ng extracting proteins from at least one set of cells; digesting the extracted proteins; derivatising the protein fragment mixture with an isotopically labelled reagent molecule; separating the protein fragment mixture by multi- dimensional chromatography; and analysing the protein fragment mixture by ma ss spectroscopy (MS) in parent ion or neutral loss scanning mode, thereby detecting or measuring the amounts of the labelled protein fragments. In a preferred embodiment, two sets of cells are combined in the method in order to compare the expression levels of two different states. Moreover, the inventi on relates to a kit for use in the present labelling method.
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