A PEPTIDE-BASED FLUORESCENCE POLARIZATION ASSAY FOR DETECTION OF ANTIBODIES TO MYCOBACTERIUM BOVIS
摘要
The present invention provides an assay for detection of M. bovis-infected animals. A tracer, comprising a peptide of M. bovis protein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific for M. bovis. The tracer comprises a peptide of M. bovis protein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for M. bovis to produce a detectable change in fluorescence polarization.
申请公布号
WO03038402(A3)
申请公布日期
2003.07.10
申请号
WO2002US34964
申请日期
2002.10.31
申请人
DIACHEMIX LLC;SURUJBALLI, OM, P.;ROMANOWSKA, ANNA;JOLLEY, MICHAEL, E.;NASIR, MOHAMMAD, SARWAR
发明人
SURUJBALLI, OM, P.;ROMANOWSKA, ANNA;JOLLEY, MICHAEL, E.;NASIR, MOHAMMAD, SARWAR