METHOD OF CALCULATING OCCURRENCE FREQUENCY OF SEQUENCE, METHOD OF CALULATING DEGREE OF ISOLATION AND METHOD OF ESTIMATING DEGREE OF ADEQUACY FOR PRIMER

摘要

It is intended to support a unique design of a primer. To calculate an indication showing the occurrence frequency of a sequence in a genome sequence, the occurrence frequencies of partial sequences having a definite length in the genome sequences are calculated. Then the occurrence of frequency of each partial sequence of the definite length is stored in a frequency/degree of isolation table (16). Concerning each partial sequence of the definite length, a degree of isolation i, which means that j mutation indicating the conversion of j bases (j<=i-1) does not occur in the genome sequence but i mutation indicating the conversion of i bases occurs in the genome sequence, is calculated. Then the degrees of isolation of the partial sequences of the definite length are stored in the frequency/degree of isolation table (16). In a visualization processing unit (18), respective bases are colored in a definite manner based on the occurrence of frequency and/or the degree of isolation of each base to give an image showing the genome sequence.