发明名称 |
AUTOMATED PROTEIN PURIFICATION IN THE MULTIWELL FORMAT BY VACUUM FILTRATION |
摘要 |
<p>The invention relates to a process and a reagent kit for obtaining clear solutions containing cell contents from biological samples in a high throughput process, comprising the steps of preparing a plurality of protein-containing solutions which contain insoluble ingredients, in separate chambers of a multi-chamber filtration unit, eliminating insoluble ingredients by filtering the solutions through the multi-chamber filtration unit with the application of a pressure differential, while preventing cross contamination between adjacent chambers by chemical and/or mechanical means, and collecting the individual filtrates separately in collecting containers, as well as a process for recovering cell contents from the clear solutions obtained. The invention further relates to the use of an apparatus for recovering clear protein-containing solutions from biological samples.</p> |
申请公布号 |
EP1206483(B1) |
申请公布日期 |
2003.07.09 |
申请号 |
EP20000949438 |
申请日期 |
2000.08.02 |
申请人 |
QIAGEN GMBH |
发明人 |
RIBBE, JOACHIM;SCHAEFER, FRANK;STEINERT, KERSTIN;LUBENOW, HELGE |
分类号 |
B01J20/281;B01D19/04;B01L3/00;C07K1/14;C07K1/34;C12M1/32;C12N9/10;C12N15/10;C12Q1/02;G01N1/34;G01N30/88;(IPC1-7):C07K1/36;B01D29/01;B01D61/18;B01L9/06 |
主分类号 |
B01J20/281 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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