Cells that lack p19ink4d and p27kip1 activity and methods of use thereof

摘要

Non-human animals that have been manipulated so as not to express a functional p19INK4d and p27KIP1 proteins are described. One particular non-human animal exemplified is a p19INK4d-double null, p27KIP1-double null mouse. Such knockout mice exhibit bradykinesia, proprioceptive abnormalities, seizure-like activity, and generally die 14-24 days after birth. In addition, mammalian cells having the p19INK4d-double null, p27KIP1-double null genotype are characterized. Methods of making and using the non-human knockout animals and cells are disclosed.