| 摘要 |
A microbial alcohol dehydrogenase (ADH) enzyme defined as in one of (1)-(4) below, is new: (1) it retains at least 95% of its activity after 30 minutes at 20-60 degrees C and can be purified to a specific activity of at least 400 U/mg; (2) it has a molecular weight of about 104 kD (gel filtration) and has Ser, Asn, Asp, Thr and/or Met in amino acid positions 1, 2, 5, 24 and/or 34, respectively; (3) it has a defined sequence of 252 amino acids given in the specification; or (4) it is encoded by a defined DNA sequence of 756 bp given in the specification. Also claimed is an isolated DNA fragment comprising the above DNA sequence. |
