LATE-PCR

摘要

A non-symmetric polymerase chain reaction (PCR) amplification method employi ng a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, th e latter in turn not being more than 25~C below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assa ys and primer or primer~and-probe sets for performing the foregoing amplifications and assays.