发明名称 |
Monoclonal antibody and method and kit for immunoassay of soluble human ST2 |
摘要 |
A method for determining a soluble human ST2 in a sample conveniently at a high sensitivity and an assay kit are provided. By an immunological method comprising a step for bringing a sample into contact with an immobilized antibody formed by binding to an insoluble support a first anti-human ST2 antibody which binds specifically to a non-denatured human ST2, a step for labelling a first reaction product generated in the previous step by reacting said first reaction product with a second anti-human ST2 antibody which binds specifically to a non-denatured human ST2 by recognizing a site different from the site on ST2 where said first anti-human ST2 antibody binds and which is labelled with a label, and a step for determining the amount of the label on said first reaction product which has been labelled, a soluble human ST2 in a sample is determined. In addition, a recombinant ST2 is employed as a standard to prepare a calibration curve, based on which the ST2 in a sample is quantified.
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申请公布号 |
US2003124624(A1) |
申请公布日期 |
2003.07.03 |
申请号 |
US20020243949 |
申请日期 |
2002.09.16 |
申请人 |
MEDICAL BIOLOGICAL LABORATORIES CO., LTD. |
发明人 |
TOMINAGA SHIN-ICHI;ARAI TAKAO;KUROIWA KENJI;OSHIKAWA KATSUHISA |
分类号 |
C07K16/28;G01N33/564;G01N33/68;(IPC1-7):G01N33/53;G01N33/542;C12N5/06;C07K16/40 |
主分类号 |
C07K16/28 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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