FERMENTATION PROCESS FOR THE PREPARATION OF L-AMINO ACIDS USING CORYNEFORM BACTERIA

摘要

The invention relates to a process for the preparation of L-amino acids in which the following steps are carried out: a) fermentation of coryneform bacteria producing the desired L-amino acid, in which at least the gene coding for a small integral C4-dicarboxylate membrane transport protein and/or the gene coding for the 2-oxoglutarate/malate translocator are attenuated; b) enrichment of the desired L-amino acid in the medium or in the cells of the bacteria; and c) isolation of the L-amino acid, and optionally bacteria are used in which other genes of the biosynthetic pathway of the desired L-amino acid are additionally enhanced, or bacteria are used in which the metabolic pathways that decrease the formation of the desired L-amino acid are at least partially switched off.