发明名称 |
Target detection method, reagent and method for detecting SNP of DNA nucleotide sequences |
摘要 |
<p>A reagent and method for detecting SNPs of DNA nucleotide sequences by using a single kind of beads of the same grain size are provided. Beads on which a probe is immobilized that hybridizes with a DNA nucleotide sequence having no SNP is mixed with beads on which another probe is immobilized that hybridizes with a DNA nucleotide sequence having SNP to make a reagent. The reagent is added to a sample solution containing a fluorescence-labeled DNA nucleotide sequence under investigation and hybridized. The beads after the reaction are flown in a flow cytometer to obtain a fluorescence intensity histogram pattern, based on which SNP of the DNA nucleotide sequence under investigation is determined.</p> |
申请公布号 |
EP1323834(A2) |
申请公布日期 |
2003.07.02 |
申请号 |
EP20020028697 |
申请日期 |
2002.12.23 |
申请人 |
HITACHI SOFTWARE ENGINEERING CO., LTD. |
发明人 |
SASAKI, KOHSUKE;FURUYA, TOMOKO |
分类号 |
G01N33/53;G01N33/483;C12N15/09;C12Q1/68;G01N21/78;G01N33/566;G01N33/58;(IPC1-7):C12Q1/68;G01N33/543 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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