REAL-TIME GENE QUANTIFICATION WITH INTERNAL STANDARD

摘要

PROBLEM TO BE SOLVED: To obtain a nucleic acid quantification kit and to provide a method for determining the initial concentration or mass fraction of a target nucleic acid present in a sample.

SOLUTION: Illustrative embodiments include real-time competitive quantitative polymerase chain reaction (PCR) to determine the copy number or mass fraction of a target nucleic acid sequence in a sample of temperature cycle of a nucleic acid sequence in the presence of a thermostable DNA polymerase and use of a thermodynamically based signal processing algorithm, with or without PCR, to provide mass fraction information.

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