METHOD FOR ASSAYING MORE THAN ONE IMMUNOLOGICAL LIGAND, AND ASSAY REAGENT AND KIT THEREFOR

摘要

An assay reagent and a kit whereby nonspecific capture of a labeled substance by a solid phase can be suppressed and more than one antibody or more than one antigen can be detected via a simple assay by using one reagent. An immunological ligand assay reagent comprising components selected from the following groups (A) and (B) at the same time is reacted with immunogical ligands to thereby form complexes, which are captured by separate solid phases (C) and analyzed based on the labeling substance contained in each complex: group (A): more than one type of immunological antiligand-nucleotide conjugates wherein antibodies having specific avidities respectively for immunological ligands of different types are bonded to nucleotides which are specific respectively for said immunological ligands and each has an arbitrarily selected base sequence; group (B): labeled substances having specific avidities respectively for the immunological antiligands of different types which are to be assayed; and group (C): solid phase-nucleotide conjugates wherein nucleotides having base sequences capable of complementarily binding respectively to the nucleotides of the group (A) are bonded to a water-insoluble carrier. Either antigens or antibodies are applicable to said immunological ligands and immunological antiligands. $$