VP7 ANTIGEN OF AFRICAN HORSE SICKNESS VIRUS(AHSV) AND DIAGNOSTIC METHOD OF AFRICAN HORSE SICKNESS(AHS) USING MONOCLONAL ANTIBODY THEREFOR

摘要

PURPOSE: A VP7 antigen of African horse sickness virus(AHSV) and a diagnostic method of African horse sickness(AHS) using a monoclonal antibody therefor are provided, thereby rapidly and accurately diagnosing AHSV. CONSTITUTION: A diagnostic method of African horse sickness(AHS) using a monoclonal antibody to VP7 antigen of African horse sickness virus(AHSV) comprises the steps of: (1) adhering a monoclonal antibody for VP7 antigen of African horse sickness virus(AHSV) to a solid support; (2) binding the VP7 antigen of AHSV with the monoclonal antibody adhered to the solid support; (3) reacting a sample containing an antibody for a VP7 protein with the VP7 antigen of AHSV; (4) reacting a secondary antibody selected from the group consisting of an enzyme conjugated antibody, a radioactive material conjugated antibody and a fluorescent material conjugated antibody, with the antibody of the step 3; and (5) quantifying the antibody in the sample, wherein the monoclonal antibody for VP7 antigen of African horse sickness virus(AHSV) is produced from a hybridoma cell(KCTC 10136BP).