| 摘要 |
Apparatus and methods for imaging multiple flourescent sample particles (210) in a flow channel (206). A flow channel defines a flow direction for samples in a flow stream (204) ahd has a viewing plane (234) transverse to the flow direction. A clear volume (226) between the illuminated flow volume and the imaging optics (208) is provided. A beam of illumination is formed as a column having a size effective to cover the viewing plane, and illuminates the flow nearly end on, or from the side. Imaging optics are arranged to view the focal plane to form an image of the multiple fluorescent sample particles in the flow stream. A camera (218) records the image formed by the imaging optics. A computer detects and enumerates the particles in the flow.
|
