发明名称 |
Method for producing a heterologous secreted protein from chinese hamster ovary cells grown on microcarriers. |
摘要 |
The present invention relates to a method for producing a heterologous secreted protein from transformed CHO cells grown on microcarriers in a serum-free cell culture medium. This method avoids a production lag phase when switching from a serum-containing to a serum-free environment, a problem observed in the mammalian adherent cell culture production of secreted proteins. |
申请公布号 |
ZA200201119(B) |
申请公布日期 |
2003.06.25 |
申请号 |
ZA20020001119 |
申请日期 |
2002.02.08 |
申请人 |
APPLIED RESEARCH SYSTEMS ARS HOLDING N.V. |
发明人 |
MARK CUNNINGHAM;TOM IHLEY |
分类号 |
C12N15/09;C07K14/715;C12N5/00;C12N5/10;C12N15/12;C12P21/02;C12P21/04;C12R1/91 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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