| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for highly accurately detecting and determining pyrophosphoric acid, to provide a method for detecting and determining nucleic acid by various nucleic acid proliferation methods utilizing the method for detecting and determining the pyrophosphoric acid, and to provide an apparatus useful for the method. SOLUTION: This method for accurately detecting and determining the pyrophosphoric acid is characterized by comprising a process for adding inosinic acid and/or xanthylic acid and a tetrazolium salt to a solution containing pyrophosphoric acid, a process for treating the pyrophosphoric acid with hypoxanthine phosphoribosyltransferase and dehydrogenase/oxidase to obtain the formazan, and a process for detecting and determining the produced formazan. COPYRIGHT: (C)2003,JPO
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