发明名称 |
Transmembrane trapping methods |
摘要 |
The present invention provides a method of cloning a protein coding for a membrane protein without the need of using anyantibody or ligand.The method of cloning a membrane protein comprises selecting a protein having a signal sequence, using a DNA coding for the signal sequence and N-terminal sequence of the protein as the reporter gene, and screening for a gene for a protein having a transmembrane domain(s).The present invention is concerned also with the CDNA obtained by using such method, a vector containing the DNA, a transformant as transformed with the vector, and the transcription product of the DNA as produced by the transformant.
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申请公布号 |
US6582905(B1) |
申请公布日期 |
2003.06.24 |
申请号 |
US19990230378 |
申请日期 |
1999.03.15 |
申请人 |
NAKAUCHI HIROMITSU;NAKAMURA YUKIO;MIYOSHI SOUSUKE;KIM DONG KU |
发明人 |
NAKAUCHI HIROMITSU;NAKAMURA YUKIO;MIYOSHI SOUSUKE;KIM DONG KU |
分类号 |
C12N15/09;C07K14/52;C07K14/705;C12N15/10;C12P21/00;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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