The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
申请公布号
WO02070755(A3)
申请公布日期
2003.06.19
申请号
WO2001US44953
申请日期
2001.11.15
申请人
THIRD WAVE TECHNOLOGIES, INC.;LYAMICHEV, VICTOR, I.;KAISER, MICHAEL, W.;LYAMICHEVA, NATASHA