METHOD FOR DETECTING IMMOBILIZATION OF PROBES AND METHOD FOR DETECTING BINDING DEGREE BETWEEN THE PROBES AND TARGET SAMPLES

摘要

PURPOSE: A method for detecting immobilization of probes and a method for detecting binding degree between the probes and target samples are provided, thereby simultaneously and accurately detecting immobilization of probe DNA and hybridization of probe DNA with target DNA. CONSTITUTION: A method for detecting immobilization of probes comprises the steps of: (1) supplying probes to a biochip with an MOSFET type sensor; (2) combining the probes with the surface of a gate electrode of the MOSFET type sensor; and (3) measuring characteristics of voltage and current passed through the gate electrode. A method for detecting binding degree between probes and target samples comprises the steps of: (1) supplying probes to a biochip with a MOSFET type sensor to combine them with the surface of a gate electrode of the MOSFET type sensor; (2) measuring characteristics of voltage and current passed through the gate electrode; (3) combining target samples with the probes; and (4) measuring characteristics of voltage and current passed through the gate electrode.