发明名称 |
ANNEALING CONTROL PRIMER AND ITS USES |
摘要 |
The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3'-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5'-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3'-end portion and said 5'-end portion comprising at least one universal base or non-discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature. |
申请公布号 |
WO03050305(A1) |
申请公布日期 |
2003.06.19 |
申请号 |
WO2002KR01781 |
申请日期 |
2002.09.19 |
申请人 |
SEEGENE, INC.;CHUN, JONG-YOON |
发明人 |
CHUN, JONG-YOON |
分类号 |
C12N15/11;C07H21/04;C12Q1/68 |
主分类号 |
C12N15/11 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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