摘要 |
The present invention relates to an oligonucleotide for analyzing a target nucleotide sequence by hybridization and its applications. The oligonucleoti de has the following general structure. 5'-Xp-Yq-Zr-3' or 5'-Zr-Yq-Xp-3' Wherei n Xp represents a first hybridization portion having a specific hybridizing nucleotide sequence substantially complementary to said target nucleotide sequence in said sample nucleic acid to hybridize therewith; Yq represents a regulator portion comprising at least two universal bases or non- discriminatory base analogs; Zr represents a second hybridization portion having a pre-selected arbitrary nucleotide sequence; p, q and r represent th e number of nucleotides; and X,Y and Z is deoxyribonucleotide or ribonucleotid e.
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