G protein coupled receptor protein, production and use thereof

摘要

Rabbit gastropyrolic part smooth muscle-derived G protein coupled receptor proteins, partial peptides thereof; and DNAs containing said protein or partial peptide-encoding DNA are provided. The receptor protein and the DNA coding for said protein can be used for {circle over (1 determination of ligands; {circle over (2 acquisition of antibody and antiserum; {circle over (3 construction of expression system for of a recombinant type receptor protein; {circle over (4 development of the receptor binding assay system using said expression system and screening of the candidate compounds for pharmaceuticals; {circle over (5conducting a drug design based upon a comparison with structurally analogous ligands and receptors; {circle over (6 preparation of probes and PCR primers for a gene diagnosis; {circle over (7 preparation of transgenic animals; and {circle over (8preparation of model patient animals deficient in the receptor protein DNA. Elucidation of the structure and property of the G protein coupled receptor is particularly related to the development of unique pharmaceuticals which act on such a system.