发明名称 |
METHOD OF FORMING COMPLEX |
摘要 |
A method of forming a complex consisting of a double-stranded nucleic acid and oligonucleotide(s) characterized by comprising: the step of mixing a double-stranded nucleic acid with at least one oligonucleotide to give a reaction mixture, wherein the oligonucleotide is a chimeric oligonucleotide containing at least a member selected from among deoxyribonucleotides and nucleotide analogs and a ribonucleotide and having a sequence substantially complementary to the base sequence of one of the strands of the double-stranded nucleic acid as described above; and the step of incubating the reaction mixture to form a complex under such conditions that the above-described double-stranded nucleic acid is not denatured.
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申请公布号 |
EP1319710(A1) |
申请公布日期 |
2003.06.18 |
申请号 |
EP20010965691 |
申请日期 |
2001.09.18 |
申请人 |
TAKARA BIO INC. |
发明人 |
SAGAWA, HIROAKI;ENOKI, TATSUJI;KOBAYASHI, EIJI;TOMONO, JUN;KATO, IKUNOSHIN |
分类号 |
C12N15/09;C12Q1/68;(IPC1-7):C12N15/09 |
主分类号 |
C12N15/09 |
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