TRANSFORMANT HAVING RECOMBINANT REPORTER GENE IN WHICH EXPRESSION IS REGULATED BY GLUCOCORTICOID AND METHOD FOR SCREENING ANALOGUE AND INHIBITOR OF GLUCOCORTICOID USING THE SAME

摘要

PURPOSE: A transformant having a recombinant reporter gene of which expression is regulated by glucocorticoid and a method for screening the analogue and inhibitor of glucocorticoid using the same are provided, thereby cheaply and rapidly screening the analogue and inhibitor of glucocorticoid. CONSTITUTION: A method for screening the analogue and inhibitor of glucocorticoid comprises the steps of: (1) preparing a recombinant reporter gene construct of which expression is regulated by glucocorticoid and a transformant containing the same; (2) treating the transformant with glucocorticoid and a testing compound and culturing the transformant; (3) separating expressed proteins from the cultured medium; (4) adding a substrate of the reporter gene to induce the enzyme-substrate reaction; and (5) measuring the luminescence level produced by the enzyme-substrate reaction, wherein the gene construct is prepared by fusion of glucocorticoid response element(GRE) and the reporter gene; the GRE has the nucleotide sequence of SEQ ID NO: 1; the reporter gene is selected from the group consisting of luciferase, alkaline phosphatase, Chloramphenicol acetyl transferase and galactosidase; the transformant is GRE luciferase-transformed mouse glioma cell; and the substrate is luciferin.