| 摘要 |
An arrangement for the detection of the fluorescence radiation of matrix-shaped specimen carriers with a large number of individual specimens, in particular, for the analysis of chemical and biological specimen carriers is disclosed. The object of the invention, to find a new possibility for detection of fluorescence radiation of matrix-shaped specimen carriers with a large number of individual specimens which permits a highly sensitive quantitative readout of the fluorescence radiation which is characteristically influenced by the individual specimen substances, is met in that, while simultaneously exciting the fluorescence radiation of a large number of substrate pixels, the transmitting optics for transmitting the fluorescence radiation emitted by individual substrate pixels to a multi-element receiver contain an imaging objective by which every substrate pixel is correlated with a determined group of receiver elements of the multi-element receiver and which is outfitted with an additional aperture stop for limiting the angular area of the recorded fluorescence radiation, so that the receiver elements which are correlated respectively with a determined substrate pixel receive substantially no fluorescent light of neighboring substrate pixels, and a darkfield illumination is provided for exciting a large number of substrate pixels with an excitation beam bundle which cuts out the aperture of the objective as well as a substantial angular area surrounding the objective aperture.
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