IDENTIFICATION OF HIGH AFFINITY MOLECULES BY LIMITED DILUTION SCREENING

摘要

Methods are disclosed for determining the relative binding affinities of molecules for their binding partner. One type of molecule is an antibody, which has an antigen binding partner. Antibodies are tested for binding against limited dilutions of antigen. Antibodies that bind to the most dilut e antigen concentrations are determined to have a higher relative binding affinity for the antigen than antibodies that only bind to more concentrated antigen preparations.