A PROCESS FOR THE PREPARATION OF A NON-TOXIC ANTHRAX VACCINE

摘要

Anthrax toxin, comprising of protective antigen (PA), lethal factor (LF) and edema factor (EF) is a major virulent factor of B.anthracis. Protective antigen, PA is the main component of all the vaccines against anthrax. The protective efficacy of PA is greatly increased if small quantities of LF or EF are incorporated into the vaccines. An ideal vaccine against anthrax should contain PA, LF and EF together, but this combination would be toxic. Therefore, the biologically inactive mutant preparations of PA, LF and EF ma y be used together for better immunoprotection. The present invention describe s the method for generation of recombinant vaccine against anthrax, comprising of non-toxic, mutant anthrax toxin proteins. The procedure involves site- directed mutagenesis of the native genes of the toxin proteins, the expressi on and purification of the mutant proteins and finally characterization of thes e proteins.