| 摘要 |
PURPOSE: A method for breeding bentgrass using Agrobacterium sp. is provided, thereby cheaply breeding the bentgrass having anticipatable character and pesticide resistance within a short period of time. CONSTITUTION: A method for breeding bentgrass using Agrobacterium sp. comprises the steps of: culturing at least one embryo developed callus of a grass seed selected from Regent, Cato and Mariner of Agrotis palustris Hud., and Tiger of Colonial bentgrass in MS medium containing proline and 2,4-D; inserting a plasmid vector containing a bar gene having pesticide resistance and GFP gene into Agrobacterium sp.; co-culturing the embryo developed callus and Agrobacterium sp. containing the plasmid vector harboring the bar gene and the GUS gene in MS medium containing 2,4-D and acetosyringone; transferring the co-cultured embryo developed callus into MS medium containing 2,4-D, PPT and cefotaxime and selecting a transgenic embryo developed callus; and regenerating the selected transgenic embryo developed callus in a medium containing PPT and cefotaxime, wherein the Agrobacterium sp. is Agrobacterium tumefaciens strains LBA4404.
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