| 摘要 |
The invention concerns a method for obtaining <i>in vitro</i> bacterial biomasses of edaphic origin comprising steps which consist in: contacting an aqueous soil suspension with a matrix substrate, so as to form a hydrated zone, or biofilm, at the surface of the substrate, similar to the one adjacent to the clay-humus complex of the soil, essentially containing the internal bacterial constituent of the soil, so as to retain at the surface of the matrix substrate, the essential part of the bacterial flora endogenous to the original edaphic media; maturing the biofilm providing oligotrophic conditions enabling the cells which constitute most of the edaphic biofilm which have colonized the matrix substrate to migrate to the supernatant liquid phase of the matrix substrate, and gradually to prevail over it; recovering and culturing the bacterial strains most prolific in rich liquid culture media, with internal character, rustic and persistent <i>in situ</i>, capable of being cultured in liquid environment, hence capable of industrial production. The resulting bacterial biomasses and strains are useful for microbiological bacterization of soils and crop residues, in particular cereal crop residues, including those of corn.
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