| 摘要 |
<p>The invention provides a method for producing a polypeptide of interest which method comprises: providing a transformed host cell which comprises a nucleotide sequence which encodes a polypeptide of interest and which directs expression of the polypeptide of interest in the transformed host cell, or a hybridoma which comprises a nucleotide sequence encoding a polypeptide of interest; providing a culture medium suitable for mammalian cell culture; and culturing the hybridoma or transformed host cell in the culture medium under conditions that allow for expression of the polypeptide of interest, wherein the culture medium (i) comprises water, a buffer, an energy source, amino acids, a lipid source or precursor, a source of iron, non-ferrous metal ions, inorganic salts, vitamins and cofactors; and (ii) is free from each of a plant-derived or animal-derived peptone, a functional protein, and a full-length polypeptide. The invention further relates to a transformed host cell and to suitable low-cost media applicable in the method of the present invention.</p> |
