发明名称 |
Oligonucleotides having A-DNA form and B-DNA form conformational geometry |
摘要 |
Modified oligonucleotides containing both A-form conformation geometry and B-from conformation geometry nucleotides are disclosed. The B-form geometry allows the oligonucleotide to serve as substrates for RNase H when bound to a target nucleic acid strand. The A-form geometry imparts properties to the oligonucleotide that modulate binding affinity and nuclease resistance. By utilizing C2' endo sugars or O4' endo sugars, the B-form characteristics are imparted to a portion of the oligonucleotide. The A-form characteristics are imparted via use of either 2'-O-modified nucleotides that have 3' endo geometries or use of end caps having particular nuclease stability or by use of both of these in conjunction with each other.
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申请公布号 |
US2003105311(A1) |
申请公布日期 |
2003.06.05 |
申请号 |
US20020098816 |
申请日期 |
2002.03.15 |
申请人 |
ISIS PHARMACEUTICALS, INC. |
发明人 |
MANOHARAN MUTHIAH;MOHAN VENKATRAMAN |
分类号 |
C12N15/09;A61K48/00;A61P35/00;A61P35/02;A61P43/00;C07H21/00;C07H21/02;(IPC1-7):A61K48/00;C07H21/04 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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