NUCLEIC ACID SEPARATION USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY

摘要

An immobilized metal affinity chromatography (IMAC) apparatus and method for separating and/or purifying compounds containing a non-shielded purine or pyrimidine moiety or group such as nucleic acid, presumably through interaction with the abundant aromatic nitrogen atoms in the purine or pyrimidine moiety. The apparatus and method can also be used to purify compounds containing purine or pyrimidine moieties where the purine and pyrimidine moieties are shielded from interaction with an IMAC ligand from compounds containing a non-shielded purine or pyrimidine moiety or group.