NONHUMAN MODEL ANIMAL UNRESPONSIVE TO IMMUNOPOTENTIATING SYNTHETIC COMPOUND

摘要

It is intended to provide a nonhuman model animal unresponsive to a syntheti c compound which lacks a gene function encoding TLR7 recognizing an immunopotentiating synthetic compound such as imidazoquinoline on its chromosome. A gene fragment of the whole gene sites including the intracellular domain and the transmembrane domain or a part thereof of a TLR 7 gene obtained from a mouse gene library is substituted by a plasmid having polyA signal and a marker gene to construct a targeting vector. Then this targeting vector is linearized and transferred into embryo stem cells. The target embryo stem cells lacking the TLR7 gene function are microinjected in to a mouse blastocyst to construct a chimeric mouse. Then this chimeric mouse i s crossed with a wild type mouse to give a heterozygote mouse. Next, the heterozygote mouse is intercrossed and thus a TLR7 knockout mouse is obtaine d.