摘要 |
The invention relates to a method for correcting the size and/or shape of a measuring volume (18) in a chemical and/or biological sample, which can be used more particularly within the field of fluorescence spectroscopy. According to said method, a measuring volume (18) disposed inside the sample (14) is imaged onto a detector device (44) with the aid of an observation lens (32). Imaging occurs via a wall (16) of a sample carrier (12) holding said sample (14). In the next step, the thickness of the sample carrier wall (16) is automatically determined. Subsequently, at least one lens (28) is automatically displaced in the direction of an optical axis of the observation lens (32) in order to correct the size and/or shape of the measuring volume (18) according to the thickness of the sample carrier wall (16). |