Integrated portable biological detection system

摘要

A system for separation of bacterial and cancer cells from peripheral human blood in microfabricated electronic chips by dielectrophoresis. The isolated cells are examined by stining the nuclei with fluorescent dye followed by laser induced fluorescence imaging. DNA and RNA is also released from the isolated cells electronically and specific marker sequences are detected by DNA amplification followed by electronic hybridization to immobilized capture probes. Efforts towards the contruction of a "laboratory-on-a-chip" system are presented which involves the selection of DNA probes, dyes, reagents and prototyping of the fully integrated portable instrument.