CULTURE MEDIUM TO IMPROVE THE PURITY OF MYOBLAST CULTURES AND METHOD USING SAME

摘要

The present invention relates to a novel culture medium composition to improve the percentage of myoblasts in a primary culture made from a muscle biopsy, which in turn permits a more successful treatment of myopathies and cardiopathies. The present invention also relates to a method to increase the preparation of myoblasts in a primary culture of mixed cells and in particular from a muscle biopsy. The invention also relates to such enriched myoblast cultures to treat myopathies and cardiopathies. In addition, the invention relates to a nutrient medium which increases the proportion of myoblasts with respect to other cell types. The present invention also relates to a myoblast basal growth medium comprising all nutrients required for growth and expansion of myoblasts wherein at least a portion of the amino acid L-valine is replaced with D-valine.