Bioactive whey protein hydrolysate free of bitter flavours wherein the enzyme used is a heat labile protease

摘要

A process for preparing a whey protein hydrolysate containing bioactive peptides is described. The process comprises hydrolyzing a whey protein isolate (WPI) with one or more enzymes characterised in that: (i) the enzyme is selected from the group consisting of Protease P6, Protease A, Protease M, Peptidase, Neutrase, Validase, AFP 2000, and any other heat labile protease; (ii) the hydrolysis is carried out at a temperature of between 30oC and 65oC at a pH of about 3.5 to 9.0 when the enzyme is a neutral protease, at a pH of about 2.5 to 6.0 where the enzyme is an acid protease and at a pH of about 5.0 to 10.0 where the enzyme is an alkaline protease; (iii) the hydrolysis is terminated when a degree of hydrolysis of no greater then about 10% has been reached; (iv) the hydrolysis is terminated by deactivating one or more of the enzymes; and (v) the conditions for step (iv) are sufficiently mild to avoid substantial denaturing of peptides or residual proteins in the hydrolysate; wherein the product produced by the process is highly soluble.