| 摘要 |
<p>An improved primer discrimination method in allele-specific primer extension performed on a DNA chip is provided. The method uses a primer, which is to be immobilized on a surface or a solid support of the DNA chip, including in a 3' portion an allele-specific nucleotide complementary to an allelic variation nucleotide of a target nucleic acid to be assayed and at least one artificial mismatch nucleotide adjacent to the allele-specific nucleotide. The accuracy in base sequence analysis and mutant genotyping can be markedly improved. The method is effective in detecting a single point mutation as well as insertion and deletion variations.</p> |
