发明名称 |
MULTIPLEX PCR |
摘要 |
Compositions and methods for nucleic acid amplification are provided that minimize the formation and amplification of spurious products, particularly in multiplex amplification reactions. Linear amplification molecules are provided. A first embodiment comprises a first common primer sequence, a first template homology region, a cleavable site, a second common primer sequence and a second template homology region. Methods employing the linear amplification molecules for nucleic acid amplification are also provided. A first embodiment comprises a template-specific hybridization, a linear amplification, a template-specific intramolecular hybridization, a strand cleavage, a second linear amplification and an exponential amplification using common primers. |
申请公布号 |
WO03044177(A2) |
申请公布日期 |
2003.05.30 |
申请号 |
WO2002US37238 |
申请日期 |
2002.11.19 |
申请人 |
PARALLELE BIOSCIENCE, INC. |
发明人 |
FAHAM, MALEK;NAMSARAEV, EUGENI, ANATOLYEVICH;WILLIS, THOMAS, DANIEL |
分类号 |
C12N15/09;C12P19/34;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|