| 发明名称 |
Method for cloning and expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli and purification of BsmBI endonuclease |
| 摘要 |
The present invention relates to recombinant DNA that encodes the BsmBI restriction endonuclease as well as BsmBI methyltransferase, expression of BsmBI restriction endonuclease and BsmBI methylase in E. coli cells containing the recombinant DNA. It also relates to a method for purification of the recombinant BsmBI restriction endonuclease.
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| 申请公布号 |
US2003100052(A1) |
申请公布日期 |
2003.05.29 |
| 申请号 |
US20010966997 |
申请日期 |
2001.09.28 |
| 申请人 |
NEW ENGLAND BIOLABS |
发明人 |
XU SHUANG-YONG;DORE ANDREW;HUME ADAM;PELLETIER JOHN;ZHOU JING |
| 分类号 |
C12N15/09;C07K14/32;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/10;C12N9/16;C12N9/22;C12N15/54;C12N15/55;C12R1/07;(IPC1-7):C12P21/02;C07H21/04;C12N1/20;C12N15/74 |
主分类号 |
C12N15/09 |
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